The mechanism of altered Rab11A movement is likely related to a decrease in dynein motors bound to Rab11A vesicles during IAV infection. Using two-color imaging we demonstrate co-transport of Rab11A and IAV vRNA in infected cells and provide direct evidence that vRNA-associated Rab11A have altered transport. Unexpectedly, infection with respiratory syncytial virus alters Rab11A motion in a manner opposite to IAV, suggesting that Rab11A is a common host component that is differentially manipulated by respiratory RNA viruses. Here, using high spatiotemporal resolution light-sheet microscopy (~1.4 volumes/second, 330 nm isotropic resolution), we quantify Rab11A and vRNA movement in live cells during IAV infection and report that IAV infection decreases speed and increases arrest of Rab11A. Rab11A-containing recycling endosomes have been identified as a platform for intracellular transport of viral RNA (vRNA). Assembly of infectious influenza A viruses (IAV) is a complex process involving transport from the nucleus to the plasma membrane. The seller's failure to submit evidence of fair market value on the date of breach did not preclude the. Upon appeal, the court held that the seller was entitled not only to the deposit, but also for actual damages. After trial, the court found that the prospective buyers had breached the agreement, but that the seller could neither recover additional damages, nor retain the deposit. The seller alleged that the buyers' failure to comply with the terms of the mortgage contingency clause precluded their proper termination of the agreement, and counterclaimed for consequential damages in excess of the liquidated amount of the deposit. In an action brought by the prospective buyers to recover a real estate deposit following their termination of the parties' purchase and sale agreement because of an inability to obtain mortgage financing, the trial court found that the clause released the prospective buyers from their obligation to purchase and that they were entitled to their deposit.
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